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mouse msc functional identification kit  (R&D Systems)


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    R&D Systems mouse msc functional identification kit
    Identification and isolation <t>of</t> <t>MSCs</t> from mouse kidneys. A: in vitro culture of MSCs isolated from adult kidney. Following retrograde perfusion of kidneys with saline buffer, cells were isolated using established methods. Cells show spindle-like shape and preferential attachment to polystyrene. B: flow cytometry was used to confirm the immunophenotype of MSCs using antibodies to Sca-1, c-kit, CD31, CD34, CD45, cytokeratin. More than 90% of cells expressed Sca-1; less than 10% of cells expressed the hematopoietic stem cell markers (c-kit, CD45, CD31, CD34). C: cells were induced to differentiate by the addition of adipogenic, osteogenic, and chondrogenic supplements. To confirm cell identity, slides were fixed and probed with polyclonal antibodies to FABP4 (adipocytes) and osteopontin (osteoblasts) and mCollagen II (chondrocytes). <t>MSC,</t> mesenchymal stem cell; Sca-1, stem cell antigen-1.
    Mouse Msc Functional Identification Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 102 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse msc functional identification kit/product/R&D Systems
    Average 93 stars, based on 102 article reviews
    mouse msc functional identification kit - by Bioz Stars, 2026-02
    93/100 stars

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    1) Product Images from "Aging phenotype(s) in kidneys of diabetic mice are p66ShcA dependent"

    Article Title: Aging phenotype(s) in kidneys of diabetic mice are p66ShcA dependent

    Journal: American Journal of Physiology - Renal Physiology

    doi: 10.1152/ajprenal.00608.2017

    Identification and isolation of MSCs from mouse kidneys. A: in vitro culture of MSCs isolated from adult kidney. Following retrograde perfusion of kidneys with saline buffer, cells were isolated using established methods. Cells show spindle-like shape and preferential attachment to polystyrene. B: flow cytometry was used to confirm the immunophenotype of MSCs using antibodies to Sca-1, c-kit, CD31, CD34, CD45, cytokeratin. More than 90% of cells expressed Sca-1; less than 10% of cells expressed the hematopoietic stem cell markers (c-kit, CD45, CD31, CD34). C: cells were induced to differentiate by the addition of adipogenic, osteogenic, and chondrogenic supplements. To confirm cell identity, slides were fixed and probed with polyclonal antibodies to FABP4 (adipocytes) and osteopontin (osteoblasts) and mCollagen II (chondrocytes). MSC, mesenchymal stem cell; Sca-1, stem cell antigen-1.
    Figure Legend Snippet: Identification and isolation of MSCs from mouse kidneys. A: in vitro culture of MSCs isolated from adult kidney. Following retrograde perfusion of kidneys with saline buffer, cells were isolated using established methods. Cells show spindle-like shape and preferential attachment to polystyrene. B: flow cytometry was used to confirm the immunophenotype of MSCs using antibodies to Sca-1, c-kit, CD31, CD34, CD45, cytokeratin. More than 90% of cells expressed Sca-1; less than 10% of cells expressed the hematopoietic stem cell markers (c-kit, CD45, CD31, CD34). C: cells were induced to differentiate by the addition of adipogenic, osteogenic, and chondrogenic supplements. To confirm cell identity, slides were fixed and probed with polyclonal antibodies to FABP4 (adipocytes) and osteopontin (osteoblasts) and mCollagen II (chondrocytes). MSC, mesenchymal stem cell; Sca-1, stem cell antigen-1.

    Techniques Used: Isolation, In Vitro, Saline, Flow Cytometry

    HG-activates ROS metabolism in cultured MSCs. Cells were loaded with mitochondrial-specific dye Mitotracker Green FM and Redox Sensor Red CC-1 WT MSCs show bright yellow orange signal due to colocalization of Mitotracker Green and RCC-1, indicative of increased mitochondrial ROS metabolism. HG, high glucose; KO, knockout; Man, mannitol; MSC, mesenchymal stem cell; NG, normal glucose; ROS, reactive oxygen species; WT, wild-type.
    Figure Legend Snippet: HG-activates ROS metabolism in cultured MSCs. Cells were loaded with mitochondrial-specific dye Mitotracker Green FM and Redox Sensor Red CC-1 WT MSCs show bright yellow orange signal due to colocalization of Mitotracker Green and RCC-1, indicative of increased mitochondrial ROS metabolism. HG, high glucose; KO, knockout; Man, mannitol; MSC, mesenchymal stem cell; NG, normal glucose; ROS, reactive oxygen species; WT, wild-type.

    Techniques Used: Cell Culture, Knock-Out

    HG-induced aging phenotypes in cultured MSCs. A: WT-MSCs exhibit increased rates of apoptosis; which is suppressed in p66 KO-MSCs. B: growth curves of WT and p66 KO-MSCs show p66 KO-MSCs in active growth phase at day 12 whereas growth of WT MSCs is suppressed by day 6. C: representative immunoblot analyses of lysates from WT MSCs and p66 KO-MSCs. The senescent proteins p53, p21, and p16INK4a are upregulated; p66 KO-MSCs show no alteration in expression levels of these proteins. Densitometric analysis of Western blots of p53 (C1), p21 (C2), and p16 (C3) is shown as bar graphs. Data are presented as mean ± SD; n = 3 in each group; (*P < 0.01, B). HG, high glucose; KO, knockout; MSC, mesenchymal stem cell; NG, normal glucose; OD405, optical density at 405 nm; WT, wild-type.
    Figure Legend Snippet: HG-induced aging phenotypes in cultured MSCs. A: WT-MSCs exhibit increased rates of apoptosis; which is suppressed in p66 KO-MSCs. B: growth curves of WT and p66 KO-MSCs show p66 KO-MSCs in active growth phase at day 12 whereas growth of WT MSCs is suppressed by day 6. C: representative immunoblot analyses of lysates from WT MSCs and p66 KO-MSCs. The senescent proteins p53, p21, and p16INK4a are upregulated; p66 KO-MSCs show no alteration in expression levels of these proteins. Densitometric analysis of Western blots of p53 (C1), p21 (C2), and p16 (C3) is shown as bar graphs. Data are presented as mean ± SD; n = 3 in each group; (*P < 0.01, B). HG, high glucose; KO, knockout; MSC, mesenchymal stem cell; NG, normal glucose; OD405, optical density at 405 nm; WT, wild-type.

    Techniques Used: Cell Culture, Western Blot, Expressing, Knock-Out

    Gene expression profiling in p66 KO-MSCs. Heat map showing gene expression pattern of WT and p66 KO MSCs at HG compared with MSCs grown under NG; red (upregulation); green (downregulation) n = 3 for each condition (A). Microarray expression analysis of p66 KO-MSCs at HG shows robust increase in IGF-1, VEGF, and HGF (B). Paracrine factors are released by MSCs at HG (C). ELISA demonstrates the presence of IGF-1, VEGF, and HGF in conditioned medium collected from p66 KO-MSCs and WT MSCs. HG, high glucose; KO, knockout; MSC, mesenchymal stem cell; NG, normal glucose; WT, wild-type.
    Figure Legend Snippet: Gene expression profiling in p66 KO-MSCs. Heat map showing gene expression pattern of WT and p66 KO MSCs at HG compared with MSCs grown under NG; red (upregulation); green (downregulation) n = 3 for each condition (A). Microarray expression analysis of p66 KO-MSCs at HG shows robust increase in IGF-1, VEGF, and HGF (B). Paracrine factors are released by MSCs at HG (C). ELISA demonstrates the presence of IGF-1, VEGF, and HGF in conditioned medium collected from p66 KO-MSCs and WT MSCs. HG, high glucose; KO, knockout; MSC, mesenchymal stem cell; NG, normal glucose; WT, wild-type.

    Techniques Used: Expressing, Microarray, Enzyme-linked Immunosorbent Assay, Knock-Out

    Gene profiling in cultured MSCs. Heat map showing expression pattern of Wnt genes in WT and p66 KO MSCs under NG and HG conditions (A); red (upregulation); green (downregulation); n = 3 for each condition. Gene expression profile (B); p66 KO-MSCs vs. WT MSCs. Representative immunoblot analysis of extracts from WT and p66 KO-MSCs showing expression levels of total β-catenin (C). n = 3. Densitometric analysis of Western blots of β-catenin/β-actin p53 (C1) is shown as bar graphs. HG, high glucose; KO, knockout; MSC, mesenchymal stem cell; NG, normal glucose; WT, wild-type.
    Figure Legend Snippet: Gene profiling in cultured MSCs. Heat map showing expression pattern of Wnt genes in WT and p66 KO MSCs under NG and HG conditions (A); red (upregulation); green (downregulation); n = 3 for each condition. Gene expression profile (B); p66 KO-MSCs vs. WT MSCs. Representative immunoblot analysis of extracts from WT and p66 KO-MSCs showing expression levels of total β-catenin (C). n = 3. Densitometric analysis of Western blots of β-catenin/β-actin p53 (C1) is shown as bar graphs. HG, high glucose; KO, knockout; MSC, mesenchymal stem cell; NG, normal glucose; WT, wild-type.

    Techniques Used: Cell Culture, Expressing, Western Blot, Knock-Out



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    Identification and isolation <t>of</t> <t>MSCs</t> from mouse kidneys. A: in vitro culture of MSCs isolated from adult kidney. Following retrograde perfusion of kidneys with saline buffer, cells were isolated using established methods. Cells show spindle-like shape and preferential attachment to polystyrene. B: flow cytometry was used to confirm the immunophenotype of MSCs using antibodies to Sca-1, c-kit, CD31, CD34, CD45, cytokeratin. More than 90% of cells expressed Sca-1; less than 10% of cells expressed the hematopoietic stem cell markers (c-kit, CD45, CD31, CD34). C: cells were induced to differentiate by the addition of adipogenic, osteogenic, and chondrogenic supplements. To confirm cell identity, slides were fixed and probed with polyclonal antibodies to FABP4 (adipocytes) and osteopontin (osteoblasts) and mCollagen II (chondrocytes). <t>MSC,</t> mesenchymal stem cell; Sca-1, stem cell antigen-1.
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    Identification and isolation <t>of</t> <t>MSCs</t> from mouse kidneys. A: in vitro culture of MSCs isolated from adult kidney. Following retrograde perfusion of kidneys with saline buffer, cells were isolated using established methods. Cells show spindle-like shape and preferential attachment to polystyrene. B: flow cytometry was used to confirm the immunophenotype of MSCs using antibodies to Sca-1, c-kit, CD31, CD34, CD45, cytokeratin. More than 90% of cells expressed Sca-1; less than 10% of cells expressed the hematopoietic stem cell markers (c-kit, CD45, CD31, CD34). C: cells were induced to differentiate by the addition of adipogenic, osteogenic, and chondrogenic supplements. To confirm cell identity, slides were fixed and probed with polyclonal antibodies to FABP4 (adipocytes) and osteopontin (osteoblasts) and mCollagen II (chondrocytes). <t>MSC,</t> mesenchymal stem cell; Sca-1, stem cell antigen-1.
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    Identification and isolation of MSCs from mouse kidneys. A: in vitro culture of MSCs isolated from adult kidney. Following retrograde perfusion of kidneys with saline buffer, cells were isolated using established methods. Cells show spindle-like shape and preferential attachment to polystyrene. B: flow cytometry was used to confirm the immunophenotype of MSCs using antibodies to Sca-1, c-kit, CD31, CD34, CD45, cytokeratin. More than 90% of cells expressed Sca-1; less than 10% of cells expressed the hematopoietic stem cell markers (c-kit, CD45, CD31, CD34). C: cells were induced to differentiate by the addition of adipogenic, osteogenic, and chondrogenic supplements. To confirm cell identity, slides were fixed and probed with polyclonal antibodies to FABP4 (adipocytes) and osteopontin (osteoblasts) and mCollagen II (chondrocytes). MSC, mesenchymal stem cell; Sca-1, stem cell antigen-1.

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Aging phenotype(s) in kidneys of diabetic mice are p66ShcA dependent

    doi: 10.1152/ajprenal.00608.2017

    Figure Lengend Snippet: Identification and isolation of MSCs from mouse kidneys. A: in vitro culture of MSCs isolated from adult kidney. Following retrograde perfusion of kidneys with saline buffer, cells were isolated using established methods. Cells show spindle-like shape and preferential attachment to polystyrene. B: flow cytometry was used to confirm the immunophenotype of MSCs using antibodies to Sca-1, c-kit, CD31, CD34, CD45, cytokeratin. More than 90% of cells expressed Sca-1; less than 10% of cells expressed the hematopoietic stem cell markers (c-kit, CD45, CD31, CD34). C: cells were induced to differentiate by the addition of adipogenic, osteogenic, and chondrogenic supplements. To confirm cell identity, slides were fixed and probed with polyclonal antibodies to FABP4 (adipocytes) and osteopontin (osteoblasts) and mCollagen II (chondrocytes). MSC, mesenchymal stem cell; Sca-1, stem cell antigen-1.

    Article Snippet: MSCs were differentiated into osteoblasts, adipocytes, and chondrocytes using mouse MSC functional identification kit (R&D Systems) as per manufacturer’s protocol.

    Techniques: Isolation, In Vitro, Saline, Flow Cytometry

    HG-activates ROS metabolism in cultured MSCs. Cells were loaded with mitochondrial-specific dye Mitotracker Green FM and Redox Sensor Red CC-1 WT MSCs show bright yellow orange signal due to colocalization of Mitotracker Green and RCC-1, indicative of increased mitochondrial ROS metabolism. HG, high glucose; KO, knockout; Man, mannitol; MSC, mesenchymal stem cell; NG, normal glucose; ROS, reactive oxygen species; WT, wild-type.

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Aging phenotype(s) in kidneys of diabetic mice are p66ShcA dependent

    doi: 10.1152/ajprenal.00608.2017

    Figure Lengend Snippet: HG-activates ROS metabolism in cultured MSCs. Cells were loaded with mitochondrial-specific dye Mitotracker Green FM and Redox Sensor Red CC-1 WT MSCs show bright yellow orange signal due to colocalization of Mitotracker Green and RCC-1, indicative of increased mitochondrial ROS metabolism. HG, high glucose; KO, knockout; Man, mannitol; MSC, mesenchymal stem cell; NG, normal glucose; ROS, reactive oxygen species; WT, wild-type.

    Article Snippet: MSCs were differentiated into osteoblasts, adipocytes, and chondrocytes using mouse MSC functional identification kit (R&D Systems) as per manufacturer’s protocol.

    Techniques: Cell Culture, Knock-Out

    HG-induced aging phenotypes in cultured MSCs. A: WT-MSCs exhibit increased rates of apoptosis; which is suppressed in p66 KO-MSCs. B: growth curves of WT and p66 KO-MSCs show p66 KO-MSCs in active growth phase at day 12 whereas growth of WT MSCs is suppressed by day 6. C: representative immunoblot analyses of lysates from WT MSCs and p66 KO-MSCs. The senescent proteins p53, p21, and p16INK4a are upregulated; p66 KO-MSCs show no alteration in expression levels of these proteins. Densitometric analysis of Western blots of p53 (C1), p21 (C2), and p16 (C3) is shown as bar graphs. Data are presented as mean ± SD; n = 3 in each group; (*P < 0.01, B). HG, high glucose; KO, knockout; MSC, mesenchymal stem cell; NG, normal glucose; OD405, optical density at 405 nm; WT, wild-type.

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Aging phenotype(s) in kidneys of diabetic mice are p66ShcA dependent

    doi: 10.1152/ajprenal.00608.2017

    Figure Lengend Snippet: HG-induced aging phenotypes in cultured MSCs. A: WT-MSCs exhibit increased rates of apoptosis; which is suppressed in p66 KO-MSCs. B: growth curves of WT and p66 KO-MSCs show p66 KO-MSCs in active growth phase at day 12 whereas growth of WT MSCs is suppressed by day 6. C: representative immunoblot analyses of lysates from WT MSCs and p66 KO-MSCs. The senescent proteins p53, p21, and p16INK4a are upregulated; p66 KO-MSCs show no alteration in expression levels of these proteins. Densitometric analysis of Western blots of p53 (C1), p21 (C2), and p16 (C3) is shown as bar graphs. Data are presented as mean ± SD; n = 3 in each group; (*P < 0.01, B). HG, high glucose; KO, knockout; MSC, mesenchymal stem cell; NG, normal glucose; OD405, optical density at 405 nm; WT, wild-type.

    Article Snippet: MSCs were differentiated into osteoblasts, adipocytes, and chondrocytes using mouse MSC functional identification kit (R&D Systems) as per manufacturer’s protocol.

    Techniques: Cell Culture, Western Blot, Expressing, Knock-Out

    Gene expression profiling in p66 KO-MSCs. Heat map showing gene expression pattern of WT and p66 KO MSCs at HG compared with MSCs grown under NG; red (upregulation); green (downregulation) n = 3 for each condition (A). Microarray expression analysis of p66 KO-MSCs at HG shows robust increase in IGF-1, VEGF, and HGF (B). Paracrine factors are released by MSCs at HG (C). ELISA demonstrates the presence of IGF-1, VEGF, and HGF in conditioned medium collected from p66 KO-MSCs and WT MSCs. HG, high glucose; KO, knockout; MSC, mesenchymal stem cell; NG, normal glucose; WT, wild-type.

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Aging phenotype(s) in kidneys of diabetic mice are p66ShcA dependent

    doi: 10.1152/ajprenal.00608.2017

    Figure Lengend Snippet: Gene expression profiling in p66 KO-MSCs. Heat map showing gene expression pattern of WT and p66 KO MSCs at HG compared with MSCs grown under NG; red (upregulation); green (downregulation) n = 3 for each condition (A). Microarray expression analysis of p66 KO-MSCs at HG shows robust increase in IGF-1, VEGF, and HGF (B). Paracrine factors are released by MSCs at HG (C). ELISA demonstrates the presence of IGF-1, VEGF, and HGF in conditioned medium collected from p66 KO-MSCs and WT MSCs. HG, high glucose; KO, knockout; MSC, mesenchymal stem cell; NG, normal glucose; WT, wild-type.

    Article Snippet: MSCs were differentiated into osteoblasts, adipocytes, and chondrocytes using mouse MSC functional identification kit (R&D Systems) as per manufacturer’s protocol.

    Techniques: Expressing, Microarray, Enzyme-linked Immunosorbent Assay, Knock-Out

    Gene profiling in cultured MSCs. Heat map showing expression pattern of Wnt genes in WT and p66 KO MSCs under NG and HG conditions (A); red (upregulation); green (downregulation); n = 3 for each condition. Gene expression profile (B); p66 KO-MSCs vs. WT MSCs. Representative immunoblot analysis of extracts from WT and p66 KO-MSCs showing expression levels of total β-catenin (C). n = 3. Densitometric analysis of Western blots of β-catenin/β-actin p53 (C1) is shown as bar graphs. HG, high glucose; KO, knockout; MSC, mesenchymal stem cell; NG, normal glucose; WT, wild-type.

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Aging phenotype(s) in kidneys of diabetic mice are p66ShcA dependent

    doi: 10.1152/ajprenal.00608.2017

    Figure Lengend Snippet: Gene profiling in cultured MSCs. Heat map showing expression pattern of Wnt genes in WT and p66 KO MSCs under NG and HG conditions (A); red (upregulation); green (downregulation); n = 3 for each condition. Gene expression profile (B); p66 KO-MSCs vs. WT MSCs. Representative immunoblot analysis of extracts from WT and p66 KO-MSCs showing expression levels of total β-catenin (C). n = 3. Densitometric analysis of Western blots of β-catenin/β-actin p53 (C1) is shown as bar graphs. HG, high glucose; KO, knockout; MSC, mesenchymal stem cell; NG, normal glucose; WT, wild-type.

    Article Snippet: MSCs were differentiated into osteoblasts, adipocytes, and chondrocytes using mouse MSC functional identification kit (R&D Systems) as per manufacturer’s protocol.

    Techniques: Cell Culture, Expressing, Western Blot, Knock-Out